An internal thermal sensor controlling temperature preference in Drosophila. Insulin-producing cells and their regulation in physiology and behavior of Drosophila. Refinement of tools for targeted gene expression in Drosophila. Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression. Sequence motifs for calmodulin recognition. Mapping neural circuits with activity-dependent nuclear import of a transcription factor. Visualization of neural activity in insect brains using a conserved immediate early gene, Hr38. Functional labeling of neurons and their projections using the synthetic activity-dependent promoter E-SARE. Permanent genetic access to transiently active neurons via TRAP: targeted recombination in active populations. Guenthner, C.J., Miyamichi, K., Yang, H.H., Heller, H.C. Targeted disruption of cocaine-activated nucleus accumbens neurons prevents context-specific sensitization. Localization of a stable neural correlate of associative memory. Reijmers, L.G., Perkins, B.L., Matsuo, N. The regulation and function of c-fos and other immediate early genes in the nervous system.
A visual motion detection circuit suggested by Drosophila connectomics.
The roles of co-transmission in neural network modulation. Nusbaum, M.P., Blitz, D.M., Swensen, A.M., Wood, D. Visualizing neuromodulation in vivo: TANGO-mapping of dopamine signaling reveals appetite control of sugar sensing. Dopaminergic modulation of sucrose acceptance behavior in Drosophila.
Genetically encoded neural activity indicators.
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Fluorescence measurement and photochemical manipulation of cytosolic free calcium. TRIC's modular design should enable optimization and adaptation to other organisms. Thus, TRIC facilitates the monitoring and manipulation of neuronal activity, especially those reflecting slow changes in physiological states that are poorly captured by existing methods. Furthermore, TRIC-induced expression of a neuronal silencer in nutrient-activated cells enhanced stress resistance, providing a proof of principle that TRIC can be used for circuit manipulation. TRIC was able to quantitatively monitor neuronal responses that changed slowly, such as those of neuropeptide F–expressing neurons to sexual deprivation and neuroendocrine pars intercerebralis cells to food and arousal. We found that in vitro assays predicted in vivo properties of TRIC and that TRIC signals in sensory systems depend on neuronal activity. Here we describe a transcriptional reporter of intracellular Ca 2+ (TRIC) in Drosophila that uses a binary expression system to report Ca 2+-dependent interactions between calmodulin and its target peptide. Intracellular Ca 2+ is a widely used neuronal activity indicator.
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